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份 库存999份
| 货号： | YB-0954 |
| 供应商： | 钰博生物 |
| 数量： | 大量 |
| 英文名： | pLVX-DD-tdTomato Reporter |
| 保质期： | 6个月 |
| 保存条件： | -20℃ |
| 规格： | 1ug/5ug |
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pLVX-DD-tdTomato Reporter |
| 质粒类型: | 慢病毒载体；无启动子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 9246 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | DD-tdTomato (C-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 慢病毒载体pLVX-DD-tdTomato-Reporter没有启动子元件； DD-tdTomato与tdTomato相比，N端融合了ProteoTuner destabilization domain (DD)去稳定结构域，导致tdTomato在细胞内 很快被蛋白酶降解，降低了背景荧光，是研究启动子活性的理想报告基因。 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 慢病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-DD-tdTomato_Reporter 9200 bp DNA circular 6-NOV-2009COMMENT Created by Clontech Laboratories Inc. http://www.clontech.comCOMMENT Cat. No. 631753,631754FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal misc_feature 5168..5759 /label=WPRE rep_origin complement(7068..7741) /label=pUC\origin CDS complement(7886..8882) /label=Amp(R) misc_binding 1303..1536 /label=RRE misc_binding 636..653 /label=PBS misc_feature 2028..2151 /label=cPPT/CTS LTR 5962..6598 /label=3'\LTR misc_feature 4026..4534 /label=PGK\Promoter misc_feature 4555..5154 /label=Puro(R) misc_feature 2195..2234 /label=MCS CDS 2247..2570 /label=DD CDS 2577..4004 /label=tdTomatoBASE COUNT 2262 a 2444 c 2487 g 2007 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg 2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag 2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg 2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag 2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc tcagatgagt gtgggtcaga 2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca 2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg 2581 tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct 2641 ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 2701 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc 2761 tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg 2821 attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2881 acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca 2941 aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca 3001 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga 3061 tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct 3121 acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca 3181 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc 3241 accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct 3301 cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg 3361 agggctccat gaacggccac gaattcgaga tcgagggcga gggcgagggc cgcccctacg 3421 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg 3481 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca 3541 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 3601 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga 3661 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga 3721 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg 3781 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga 3841 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc 3901 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg 3961 gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaggcg gccgcgactc 4021 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt 4081 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac 4141 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac 4201 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca 4261 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa 4321 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca 4381 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg 4441 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc 4501 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc 4561 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 4621 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 4681 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 4741 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 4801 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 4861 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 4921 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4981 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 5041 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 5101 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt 5161 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5221 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5281 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5341 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5401 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5461 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 5521 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 5581 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 5641 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 5701 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 5761 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca 5821 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca 5881 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 5941 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc 6001 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca 6061 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag 6121 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc 6181 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta 6241 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga 6301 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact 6361 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc 6421 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 6481 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 6541 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 6601 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 6661 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat 6721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 6781 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 6841 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 6901 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 6961 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 7021 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 7081 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 7141 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 7201 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 7261 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 7321 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 7381 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 7441 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 7501 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7561 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7621 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7681 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7741 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7801 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7861 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7921 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7981 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 8041 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 8101 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 8161 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 8221 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 8281 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 8341 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 8401 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 8461 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 8521 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8581 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8641 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8701 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8761 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8881 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat 8941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 9001 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat 9061 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta 9121 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata 9181 tgtactacaa acttagtagt
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