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份 库存999份
| 货号： | YB-10317 |
| 供应商： | 钰博生物 |
| 数量： | 大量 |
| 英文名： | pET-Cas9-NLS-6xHis |
| 保质期： | 6个月 |
| 保存条件： | -20℃ |
| 规格： | 1ug/5ug |
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pET-Cas9-NLS-6xHis |
| 质粒类型: | CRISPR/Cas9载体；细菌基因敲除载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 插入位点: | 限制性内切酶，多克隆位点 |
| 启动子: | lac/T7 |
| 载体大小: | 9265 bp |
| 5' 测序引物及序列: | T7 forward：5’TAATACGACTCACTATAGGG 3’ |
| 3' 测序引物及序列: | T7 Terminal：5’GCTAGTTATTGCTCAGCGG 3’ |
| 载体标签: | NLS 、 His tag (C-ter） |
| 载体抗性: | Ampicillin |
| 克隆菌株: | Mach1 |
| 宿主细胞（系）: | 细菌，如大肠杆菌 |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 9265 bp ds-DNA circular SYN 26-JUL-2016DEFINITION Bacterial vector for regulated expression of S. pyogenes Cas9 with a C-terminal nuclear localization signal and 6xHis tag.ACCESSION .VERSION .KEYWORDS pET-Cas9-NLS-6xHisSOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 9265) AUTHORS Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR. TITLE Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. JOURNAL Nat. Biotechnol. 2015;33:73-80. PUBMED 25357182REFERENCE 2 (bases 1 to 9265) AUTHORS Liu Lab / Addgene #62933 TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 http://www.snapgene.comFEATURES Location/Qualifiers source 1..9265 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" protein_bind 3..27 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 42..64 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 71..4174 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="Cas9" /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 4175..4195 /codon_start=1 /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /translation="PKKKRKV" CDS 4205..4222 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" terminator 4307..4354 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 4391..4846 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(4961..5821) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5822..5914) /gene="bla" /note="AmpR promoter" rep_origin 5995..6583 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7013..7204) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" CDS complement(7776..8858) /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" promoter complement(8859..8936) /gene="lacI" /note="lacI promoter" promoter 9249..2 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase"ORIGIN 1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 61 gagatatacc atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg 121 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa 181 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga 241 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa 301 tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt 361 ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc 421 tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca 481 tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc 541 cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga 601 taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga 661 agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag 721 taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt 781 atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga 841 tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa 901 tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc 961 agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct 1021 atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc 1081 tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa 1141 cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa 1201 accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga 1261 tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg 1321 tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg 1381 tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg 1441 tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa 1501 ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa 1561 ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta 1621 ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc 1681 agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg 1741 aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag 1801 tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt 1861 gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga 1921 ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa 1981 aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac 2041 tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa 2101 aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat 2161 ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg 2221 cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat 2281 tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga 2341 aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc 2401 gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa 2461 agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca 2521 aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga 2581 tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt 2641 aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa 2701 aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt 2761 tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat 2821 caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag 2881 tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac 2941 cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga 3001 gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt 3061 gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga 3121 tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt 3181 cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat 3241 tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg 3301 gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa 3361 aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga 3421 caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc 3481 aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt 3541 aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa 3601 tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa 3661 actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc 3721 cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata 3781 tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt 3841 gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc 3901 taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca 3961 tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa 4021 tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac 4081 gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga 4141 aacacgcatt gatttgagtc agctaggagg tgaccccaag aagaagagga aggtgatgga 4201 taagcatcac caccaccatc actaatgata atttgaacgc cagcacatgg actcgtctac 4261 tagcgcagct taattaacct aggctgctgc caccgctgag caataactag cataacccct 4321 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt 4381 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 4441 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 4501 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 4561 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 4621 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 4681 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 4741 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 4801 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttctgg cggcacgatg 4861 gcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 5041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 5101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 5161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 5221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 5281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 5341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 5581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5821 tactcttcct ttttcaatca tgattgaagc atttatcagg gttattgtct catgagcgga 5881 tacatatttg aatgtattta gaaaaataaa caaataggtc atgaccaaaa tcccttaacg 5941 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 6001 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 6061 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 6121 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 6181 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 6241 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 6301 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 6361 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 6421 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 6481 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 6541 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 6601 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 6661 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 6721 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta 6781 ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca 6841 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg 6901 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 6961 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 7021 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt 7081 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa 7141 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg 7201 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac 7261 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt 7321 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc 7381 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg 7441 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg 7501 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc 7561 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc 7621 gccagcctag ccgggtcctc aacgacagga gcacgatcat gctagtcatg ccccgcgccc 7681 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct 7741 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 7801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 7861 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 7921 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 7981 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg 8041 tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt 8101 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc 8161 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct 8221 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc 8281 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga 8341 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc 8401 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg 8461 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga 8521 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg 8581 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc 8641 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt 8701 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt 8761 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg 8821 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc 8881 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg 8941 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag 9001 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc 9061 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg 9121 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt 9181 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc 9241 cgcgaaatta atacgactca ctata
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