货号：	A07469
供应商：	美国ATCC
数量：	1000
英文名：	ATCC7469
保质期：	2年
保存条件：	2-8℃
规格：	株

ATCC7469菌株

ATCC7469标准菌株基本信息

出品公司:	ATCC
菌种名称:	ATCC7469, ATCC 7469
菌种又名:	[BUCSAV 227, M. Rogosa V300, M.E. Sharpe H2, NCDO 243, NCIB 6375, NCIB 8010, NCTC 6375, NRC 488, P.A. Hansen 300, R.P. Tittsler 300]
菌株类型:	Lactobacillus rhamnosus (Hansen) Collins et al.鼠李糖乳杆菌

产品目录号:	7469
其他保藏库编号:	NBRC3425=ATCC7469
培养基:	乳酸杆菌琼脂/肉汤培养基
生长条件:	37 ℃,5%二氧化碳
生物安全等级:	1
模式菌株:	是
应用:	叶酸含量测定，烟酰胺烟酰胺的测定，核黄素维生素B2的测定，生产叶酸转运蛋白，食品检验
菌株特点:	ATCC7469是鼠李糖乳杆菌。 ATCC 7469应用于叶酸含量测定，烟酰胺烟酰胺的测定，核黄素维生素B2的测定，生产叶酸转运蛋白，食品检验。 ATCC 7469能够在乳酸杆菌琼脂/肉汤培养基中生长。 Nucleotide (GenBank) : AF192553 Lactobacillus rhamnosus dlt operon, complete sequence. Nucleotide (GenBank) : L08854 Lactobacillus casei valyl-tRNA synthetase gene, complete cds. Nucleotide (GenBank) : J05221 L.carei folylpoly-gamma-glutamate synthetase gene, complete cds. Nucleotide (GenBank) : AF323539 Lactobacillus rhamnosus strain ATCC 7469 glycosyltransferase gene, partial cds. Nucleotide (GenBank) : AF323528 Lactobacillus rhamnosus strain ATCC 7469 priming glycosyltransferase gene, partial cds. Nucleotide (GenBank) : U43894 Lactobacillus casei dlt operon DltA, DltB, and DltC genes, complete cds, and DltD gene, partial cds. Nucleotide (GenBank) : M83993 Lactobacillus casei D-alanine-activating enzyme gene, complete cds and a potential basic membrane protein (involved in D-ala-LTA biosynthesis) gene, 5' end. Nucleotide (GenBank) : AF015453 Lactobacillus rhamnosus 6-phospho-beta-glucosidase homolog gene, partial cds; GNTR transcriptional regulator homolog and surface located protein genes, complete cds.
参考文献:	Henderson GB, et al. Kinetic evidence for two interconvertible forms of the folate transport protein from Lactobacillus casei. J. Bacteriol. 163: 1147-1152, 1985. PubMed: 3928596 Colwell RR, Liston J. Taxonomic relationships among the pseudomonads. J. Bacteriol. 82: 1-14, 1961. PubMed: 13694873 Biochem. J. 69: 403, 1958. Biochim. Biophys. Acta 54: 614, 1961. Biochim. Biophys. Acta 59: 273, 1962. Biochim. Biophys. Acta 71: 454, 1963. Ikawa M. Nature of the lipids of some lactic acid bacteria. J. Bacteriol. 85: 772-781, 1963. PubMed: 14044942 Sharpe ME. A serological classification of lactobacilli. J. Gen. Microbiol. 12: 107-122, 1955. PubMed: 14354139 Davis GH. The classification of Lactobacilli from the human mouth. J. Gen. Microbiol. 13: 481-493, 1955. PubMed: 13278499 Sharpe ME, Wheater DM. Lactobacillus helveticus. J. Gen. Microbiol. 16: 676-679, 1957. PubMed: 13439152 Clin. Chem. 5: 275, 1959. J. Appl. Bacteriol. 18: 274, 1955. J. Biol. Chem. 157: 303, 1945. Arch. Biochem. 16: 357-360, 1948. Nature 149: 696, 1942. . . J. Clin. Pathol. 14: 335, 1961. . . Zentralbl. Bakteriol., II Abt. 111: 250, 1958. Abo-Elnaga IG, Kandler O. Zur taxonomier der Gattung Lactobacillus Beijerinck. I. Das subgenus Streptobacterium Orla-Jensen. Zentralbl. Bakteriol., II Abt. 119: 1-36, 1965. Rogosa M, et al. Species differentiation of oral lactobacilli from man including description of Lactobacillus salivarius nov. spec. and Lactobacillus cellobiosus nov. spec.. J. Bacteriol. 65: 681-699, 1953. PubMed: 13069442 Birnbaum J, Lichstein HC. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92: 925-930, 1966. PubMed: 5926760 . . J. Gen. Microbiol. 37: 433-438, 1964. Brown JP, VanDemark PJ. Respiration of Lactobacillus casei. Can. J. Microbiol. 14: 829-835, 1968. PubMed: 4385940 Benito de Cardenas IL, et al. Utilization of pyruvate in Lactobacillus casei subsp. rhamnosus ATCC 7469. Curr. Microbiol. 15: 259-264, 1987. Collins MD, et al. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and lactobacillus rhamnosus sp. nov., comb. nov.. Int. J. Syst. Bacteriol. 39: 105-108, 1989. Doelle HW, Manderson GJ. Preparation of extracts of culture liquids for gas-chromatographic determination of acidic fermentation products. Antonie van Leeuwenhoek 35: 467-478, 1969. PubMed: 5311575 . Type strains of Lactobacillus species. Rockville, MD: ATCC; 1960. AOAC International Vitamin assays, microbiological method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 960.46. AOAC International Folic acid (pteroylglutamic acid) in infant formula, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 992.05. Crowley PJ, et al. Genetic and physiologic analysis of a formyl-tetrahydrofolate synthetase mutant of Streptococcus mutans. J. Bacteriol. 179: 1563-1572, 1997. PubMed: 9045814 Debabov DV, et al. The D-Alanyl carrier protein in Lactobacillus casei: cloning, sequencing, and expression of dltC. J. Bacteriol. 178: 3869-3876, 1996. PubMed: 8682792 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Girdwood RH. The microbiological assay of vitamins and amino acids. J. Med. Lab. Technol. 20: 26-33, 1963. PubMed: 13948188 type strain Total Folates in Cereals and Cereal Foods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 2004.05. Total folate in cereal products-microbiological assay using trienzyme extraction.. St. Paul, MN, 2000 Foodstuffs --- Determination of folate by microbiological assay. London, UK:British Standards Institution;British Standard BS EN 14131:2003. ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions) Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM. Collins MD, et al. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans, and lactobacillus rhamnosus sp. nov., comb. nov.. Int. J. Syst. Bacteriol. 39: 105-108, 1989. . Type strains of Lactobacillus species. Rockville, MD: ATCC; 1960. Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

ATCC7469菌株：由一个独立分离的单细胞系培养而成的纯遗传型群体及其一切后代，一种微生物的每一个不同来源的纯培养物均可称为该菌种的一个菌株。

标准菌株的培养传代
长久保存的菌种在启用时，要经移种至适宜的培养基上进行培养，待生长后再行移种，如此连续2～3 次，甚至多次，直至出现典型的形态和生化特征为止。
保存的菌种须按规定时间进行传代，以保证菌株的活力，菌株的传代次数（自原始菌种冻干粉起）一般不得超过5 次。
在检查或保存菌种过程中，遇有形态构造上有变异或污染菌等情况，需进行菌种移植分离培养以得到纯菌。
移植传代时须核对编号、传代次数、传代日期、所用培养基等并记录。每次移植传代后，要与原种的编号、名称核对，检查培养特征无误时方可再继续保存。
传代次数必须控制，传代次数越多，突变的机率越大。因此要尽量少传代，必须根据菌种保存情况规定最多传代次数。
标准菌株的使用
每次使用的ATCC7469菌株必须是培养后健康、生命力强、无变异的菌种。每次使用菌种均须作记录。
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4. ATCC7469菌株产品种类齐全：25个系统共150多种细胞，满足不同客户的要求。细胞经过严格的质控，纯度可达98％。常用产品备有现货。其产品类型如下：消化系统细胞株，呼吸系统细胞株，泌尿系统细胞株生殖系统细胞株血液循环系统内皮细胞细胞株神经系统及其他细胞株，动物细胞株和菌株
5.对于部分难培养的细胞我公司可代复苏细胞，并提供技术支持。
6.经验丰富：我公司是国内专业化的细胞销售公司，具有十余年细胞产品销售经验，有专业的销售团队和技术人员。公司的每一位员工都将竭诚为您服务。

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