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| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pGL4.79 ; pGL4.79[hRluc/Neo] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 4879 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRluc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.79[hRluc/Neo]载体是信号通路报告载体；无启动子；含海肾荧光素酶报告基因hRluc。 |
| 产品目录号: | ZY6971 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 货号： | ZY6971 |
| 供应商： | 泽叶生物 |
| 数量： | 20 |
| 英文名： | pGL4.79[hRluc/Neo] |
| 保质期： | 三年 |
| 保存条件： | -20℃低温保存 |
pGL4.79载体基本信息
pGL4.79载体质粒图谱和多克隆位点信息
pGL4.79载体简介
The pGL4.79[hRluc/Neo] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.79[hRluc/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.
pGL4.79载体序列
LOCUS DQ188843 4879 bp DNA circular SYN 16-NOV-2005DEFINITION Renilla luciferase reporter vector pGL4.79[hRluc/Neo], complete sequence.ACCESSION DQ188843VERSION DQ188843.1 GI:76364302KEYWORDS .SOURCE Renilla luciferase reporter vector pGL4.79[hRluc/Neo] ORGANISM Renilla luciferase reporter vector pGL4.79[hRluc/Neo] other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 4879) AUTHORS Stecha,P., Paguio,A. and Chauvin,F. TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega Corporation JOURNAL UnpublishedREFERENCE 2 (bases 1 to 4879) AUTHORS Stecha,P., Paguio,A. and Chauvin,F. TITLE Direct Submission JOURNAL Submitted (31-AUG-2005) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USAFEATURES Location/Qualifiers source 1..4879 /organism="Renilla luciferase reporter vector pGL4.79[hRluc/Neo]" /mol_type="other DNA" /db_xref="taxon:346090" misc_feature 1..70 /note="multiple cloning region" CDS 100..1035 /codon_start=1 /product="synthetic Renilla luciferase" /protein_id="ABA41671.1" /db_xref="GI:76364303" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" regulatory 726..4879 /regulatory_class="polyA_signal_sequence" /note="synthetic poly(A) signal/transcriptional pause site" regulatory 1336..1754 /regulatory_class="promoter" /note="SV40 early enhancer/promoter" CDS 1779..2573 /note="neomycin resistance" /codon_start=1 /product="neomycin phosphotransferase" /protein_id="ABA41672.1" /db_xref="GI:76364304" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 2598..2646 /regulatory_class="polyA_signal_sequence" /note="synthetic poly(A) signal" primer_bind 2713..2732 /note="Reporter Vector primer 4 (RVprimer4) binding region" rep_origin 2970 /note="ColEI-derived plasmid replication origin" gene complement(3761..4621) /gene="bla" CDS complement(3761..4621) /gene="bla" /note="ampicillin resistance" /codon_start=1 /product="beta-lactamase" /protein_id="ABA41673.1" /db_xref="GI:76364305" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" primer_bind 4828..4847 /note="Reporter Vector primer 3 (RVprimer3) binding region"ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc 61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac 121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg 181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg 241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc 301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc 361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg 421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt 481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg 541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag 601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca 661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag 721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag 781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc 841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc 901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc 961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg 1021 aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga 1081 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 1141 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 1201 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 1261 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg 1321 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt 1381 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa 1441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 1501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 1561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 1621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 1681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 1741 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gatcgaacaa gacggcctcc 1801 atgctggcag tcccgcagct tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga 1861 ccatcggatg tagcgatgcg gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt 1921 tcgtgaagac cgacctgagc ggcgccctga acgagcttca agacgaggct gcccgcctga 1981 gctggctggc caccaccggc gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg 2041 gccgggactg gctgctgctg ggcgaggtcc ctggccagga tctgctgagc agccaccttg 2101 cccccgctga gaaggtttct atcatggccg atgcaatgcg gcgcctgcac accctggacc 2161 ccgctacctg ccccttcgac caccaggcta agcatcggat cgagcgtgct cggacccgca 2221 tggaggccgg cctggtggac caggacgacc tggacgagga gcatcagggc ctggcccccg 2281 ctgaactgtt cgcccgactg aaagcccgca tgccggacgg tgaggacctg gttgtcacac 2341 acggagatgc ctgcctccct aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg 2401 actgcggtcg cctaggagtt gccgaccgct accaggacat cgccctggcc acccgcgaca 2461 tcgctgagga gcttggcggc gagtgggccg accgcttctt agtcttgtac ggcatcgcag 2521 ctcccgacag ccagcgcatc gccttctacc gcttgctcga cgagttcttt taatgatcta 2581 gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt 2641 tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac ccagtcagct 2701 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca 2761 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg 2821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 2881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 2941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 3001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 3061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 3121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 3181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 3241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 3301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 3361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 3421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 3481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 3541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 3601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 3661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 3721 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg 3781 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg 3841 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc 3901 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg 3961 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg 4021 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg 4081 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt 4141 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc 4201 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac 4261 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa 4321 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac 4381 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt 4441 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc 4501 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa 4561 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca 4621 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag 4681 gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta 4741 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc 4801 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca 4861 ggtgccagaa catttctct
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