Bovine NADH-ubiquinone oxidoreductase chain 1 (MT-ND1) ELISA Kit

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单价: 1900.00
品牌: Abebio
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更新: 2020-11-02
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货号: AE32410BO
样本: Serum, Plasma, Other biological fluids
标记物: P03887
适应物种: Bovine (Bos taurus; Cattle)
应用: Quantitative
检测方法: Sandwich
检测限: Request Information
供应商: 华夏远洋
数量: 999
规格: 96T/48T
简写:MT-ND1
别名:MTND1; NADH dehydrogenase subunit 1
检测范围:Request Information
产品概述:
特异性:This assay has high sensitivity and excellent specificity for detection of Bovine MT-ND1. No significant cross-reactivity or interference between Bovine MT-ND1 and analogues was observed.
回收率:Matrices listed below were spiked with certain level of recombinant Bovine MT-ND1 and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine MT-ND1 in samples.
线性:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine MT-ND1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理:This assay employs a two-site sandwich ELISA to quantitate MT-ND1 in samples. An antibody specific for MT-ND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
温馨提示:不可用于临床治疗。