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份 库存999份
| 货号： | AE34875PI |
| 样本： | Serum, Plasma, Other biological fluids |
| 适应物种： | Pig (Sus scrofa; Porcine) |
| 应用： | Quantitative |
| 检测方法： | Sandwich |
| 检测限： | 89.3 pg/mL |
| 供应商： | 华夏远洋 |
| 数量： | 999 |
| 规格： | 96T/48T |
别名：N/A
检测范围：246.9-20000 pg/mL
产品概述：Leukotriene B4 is a leukotriene involved in inflammation. It is produced from leukocytes in response to inflammatory mediators and is able to induce the adhesion and activation of leukocytes on the endothelium, allowing them to bind to and cross it into the tissue. In neutrophils, it is also a potent chemoattractant, and is able to induce the formation of reactive oxygen species and the release of lysosome enzymes by these cells.Leukotriene B4 is a potent pro-inflammatory molecule that belongs to a family of eicosanoid lipid mediators. It is synthesized from arachidonic acid that is generated from nuclear membrane phospholipid. In general, and upon cell activation, phospholipase A2 is directed to the nuclear membrane. Here, it generates arachidonic acid that is bound by one 25 kDa membrane-bound protein termed Five-Lipooxygenase Activating Protein (FLAP).
特异性：This assay has high sensitivity and excellent specificity for detection of Pig LT-B4. No significant cross-reactivity or interference between Pig LT-B4 and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Pig LT-B4 and the recovery rates were calculated by comparing the measured value to the expected amount of Pig LT-B4 in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig LT-B4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate LT-B4 in samples. An antibody specific for LT-B4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLT-B4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LT-B4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LT-B4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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