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份 库存999份
| 货号： | AE17201HU |
| 样本： | Serum, Plasma, Other biological fluids |
| 标记物： | Q15582 |
| 适应物种： | Human (Homo sapiens) |
| 应用： | Quantitative |
| 检测方法： | Sandwich |
| 检测限： | Request Information |
| 供应商： | 华夏远洋 |
| 数量： | 999 |
| 规格： | 96T/48T |
别名：BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1; RGD-containing collagen-associated protein|kerato-epithelin
检测范围：Request Information
产品概述：TGF-β is capable of producing a variety of effects and virtually all cell types respond to this factor in some way. The inappropriate presence of active TGF-β1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-β is secreted by cells in the form of an inactive complex. This complex consists of TGF-β1 associated non-covalently with a protein designated the latency associated peptide (LAP).TGF-β1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-β1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-β1 inactive and inaccessible to anti-TGF-β antibodies.
特异性：This assay has high sensitivity and excellent specificity for detection of Human TGFBI. No significant cross-reactivity or interference between Human TGFBI and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Human TGFBI and the recovery rates were calculated by comparing the measured value to the expected amount of Human TGFBI in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human TGFBI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate TGFBI in samples. An antibody specific for TGFBI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFBI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFBI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFBI bound in the initial step. The color development is stopped and the intensity of the color is measured.
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