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份 库存999份
| 货号： | AE34900HU |
| 样本： | Serum, Plasma, Other biological fluids |
| 标记物： | Q86X29 |
| 适应物种： | Human (Homo sapiens) |
| 应用： | Quantitative |
| 检测方法： | Sandwich |
| 检测限： | 0.057 ng/mL |
| 供应商： | 华夏远洋 |
| 数量： | 999 |
| 规格： | 96T/48T |
别名：ILDR3; LISCH7; MGC10659; MGC48312; MGC48503; LISCH protein|immunoglobulin-like domain containing receptor 3|lipolysis-stimulated receptor|lipolysis-stimulated remnant|liver-specific bHLH-Zip transcr
检测范围：0.156-10 ng/mL
产品概述：LSR is a multimeric protein complex in the liver that undergoes conformational changes upon binding of free fatty acids, thereby revealing a binding site (s) that recognizes both apoB and apoE. Complete inactivation of the LSR gene is embryonic lethal in mice.LSR cooperates with the LDL receptor in the final hepatic processing of apoB-containing lipoproteins and represents a novel therapeutic target for the treatment of hyperlipidemia associated with obesity and atherosclerosis. The lipolysis-stimulated receptor (LSR) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids. Antibodies inhibiting LSR functions showed that the receptor is a heterotrimer or tetramer consisting of 68-kDa (alpha) and 56-kDa (beta) subunits associated through disulfide bridges.
特异性：This assay has high sensitivity and excellent specificity for detection of Human LSR. No significant cross-reactivity or interference between Human LSR and analogues was observed.
回收率：Matrices listed below were spiked with certain level of recombinant Human LSR and the recovery rates were calculated by comparing the measured value to the expected amount of Human LSR in samples.
线性：The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human LSR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
稳定性：The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
测试原理：This assay employs a two-site sandwich ELISA to quantitate LSR in samples. An antibody specific for LSR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLSR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LSR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LSR bound in the initial step. The color development is stopped and the intensity of the color is measured.
温馨提示：不可用于临床治疗。
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