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| 货号： | DN25-10MG |
| 供应商： | 上海善然生物科技有限公司 |
| 数量： | 现货 |
| 保存条件： | 低温 |
| 规格： | DN25-10MG |
| A9539-250G | 琼脂糖 |
| T1503-5KG | Tris 碱 |
| E5134-5KG | EDTA-Na2 |
| 52365-250G | CTAB |
| 62476-500G-F | 氯化锂 |
| 31650-250G | 氯化锌 |
| PVP40-500G | PVP40 |
| S5881-1KG | 颗粒氢氧化钠 |
| 217263-250G | 硫代硫酸钠 |
| 60489-250G-F | 过硫酸钾 |
| H3506-500MG | 透明质酸酶 |
| P4937-100ML | 细胞分离液 |
| E1644-100G | EDTA-Na2 |
产品名称
Analysis Note
Protein determined by biuret.
Application
用于从蛋白质样品中除去 DNA。
DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.1 It has also been used along with trypsin for the preparation of single cell suspension from rat testes.2
Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Preparation Note
10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration.
Derived from New Zealand-sourced pancreas
Unit Definition
在 pH 5.0 和 25°C 下以 I 型或 III 型 DNA 为底物，一个 Kunitz 单位每分钟产生的 ΔA260 为 0.001。[Mg2+] = 4.2 mM。
Physical form
Crude preparation, contains calcium chloride
Biochem/physiol Actions
DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+.3 Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8.4 DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found.5 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity.
性质
| Related Categories | 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Enzyme Class Index, |
| Quality Level | PREMIUM |
| form | lyophilized powder |
| mol wt | mol wt ~31 kDa |
| composition | Protein, ≥85% |
| solubility | 0.15 M NaCl: soluble5.0 mg/mL, hazy |
| Featured Industry | Diagnostic Assay Manufacturing Diagnostic Assay Manufacturing |
| foreign activity | RNase ≤0.02% |
[VIP第1年] 指数:2
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