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份 库存999份
| 货号： | GN1214 |
| 供应商： | 上海盖宁生物 |
| 数量： | 现货 |
| 英文名： | pET-30c(+) |
| 保质期： | 三年 |
| 保存条件： | 常温 |
| 规格： | 5ug质粒 |
| 别名: | pET30c, pet 30c |
|---|---|
| 质粒类型: | 大肠杆菌表达载体 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5423 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-His, N-S, N-Thrombin, N-EK, C-His |
| 载体抗性: | Kanamycin (卡那霉素) |
| 备注: | N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| 1214 | pET-30c(+) | 5ug质粒 | ¥1000.00 |
质粒图谱
载体描述
The pET-30a-c(+) vectors carry an N-terminal His• Tag®/ thrombin/ S• Tag™/ enterokinase configuration plus an optional C-terminal His• Tag sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
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