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份 库存999份
| 货号： | ab204524 |
| 样本： | Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma |
| 标记物： | 小鼠Heme Oxygenase 1 ELISA试剂盒 |
| 应用： | Sandwich (quantitative) |
| 检测方法： | 比色法 |
| 检测限： | 78.1 pg/ml - 10000 pg/ml 灵敏度：10 pg/ml |
| 规格： | 96t |
- 产品名称小鼠Heme Oxygenase 1 ELISA试剂盒SimpleStep
- 检测方法Colorimetric
- 精确度
批次内
样品 n Mean SD CV% Overall 5 2.4% 批次间
样品 n Mean SD CV% Overall 3 2.6% - 样品类型 Cell culture supernatant, Urine, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
- 检测类型Sandwich (quantitative)
- 灵敏度 10 pg/ml
- 范围 78.1 pg/ml - 10000 pg/ml
- 复检
特定样本复检结果
样品类型 平均% 范围 Urine 105 102% - 111% Serum 96 94% - 98% Cell culture media 112 108% - 119% Heparin Plasma 88 86% - 91% EDTA Plasma 89 87% - 91% Citrate Plasma 95 87% - 108% - 检测时间 1h 30m
- 实验步骤One step assay
- 种属反应性 与反应: Mouse
不与反应: Rabbit, Guinea pig, Cow, Dog, Human, Pig - 产品概述
Abcam’s Heme Oxygenase 1 (HO 1) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Heme Oxygenase 1 protein in mouse serum, plasma, urine, cell culture supernatant, cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in Sample Diluent NS: 10 pg/mL
Samples diluted in 1X Cell Extraction Buffer PTR: 17 pg/mL
- 说明
Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 47% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.
- 经测试应用Sandwich ELISA
- 平台Microplate (12 x 8 well strips)
性能
- 存放说明Store at +4°C. Please refer to protocols.
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组件 1 x 96 tests 10X Mouse HO 1 Capture Antibody 1 x 600µl 10X Mouse HO 1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Mouse HO 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml
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