货号: | IHUIGGKT |
供应商: | 上海钰博生物科技有限公司 |
数量: | 大量 |
英文名: | Human IgG ELISA Kit |
保质期: | 1年 |
保存条件: | -2-8℃ |
规格: | 盒 |
Human IgG ELISA Kit
Product Description
Human IgG ELISA Kit
This human immunoglobulin G antigen assay is intended for the quantitative determination of total human IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses.
Human Immunoglobulin G (IgG) is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.
Human IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human IgG. Color development is proportional to the concentration of IgG in the samples.
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温馨提示:不可用于临床治疗。