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份 库存999份
| 货号： | YB-10204 |
| 供应商： | 钰博生物 |
| 数量： | 大量 |
| 英文名： | pEarleyGate101 |
| 保质期： | 6个月 |
| 保存条件： | -20℃ |
| 规格： | 1ug/5ug |
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pEarleyGate101； pEarleyGate 101 |
| 质粒类型: | Gateway载体；植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | Gateway |
| 启动子: | 35S |
| 载体大小: | 12454 bp |
| 5' 测序引物及序列: | 35S promoter：CTATCCTTCGCAAGACCCTTC |
| 3' 测序引物及序列: | -- |
| 载体标签: | 黄色荧光蛋白EYFP、HA (C ter) |
| 载体抗性: | 卡那霉素、氯霉素（仅空载体） |
| 筛选标记: | 草丁膦（磷丝菌素）phosphinothricin、 |
| 克隆菌株: | DB3.1菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEarleyGate 101 12454 bp ds-DNA circular SYN 26-JUL-2016DEFINITION Gateway(R)-compatible plant transformation vector with YFP and HA C-terminal tags.SOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 12454) AUTHORS Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS. TITLE Gateway-compatible vectors for plant functional genomics and proteomics. JOURNAL Plant J. 2006;45:616-29. PUBMED 16441352REFERENCE 2 (bases 1 to 12454) AUTHORS Pikaard Lab TITLE Direct Submission JOURNAL Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector NTI(R) format http://www.snapgene.comCOMMENT The sequence was corrected by inserting a G at position 8562.COMMENT VNTDATE|659923200|COMMENT VNTDBDATE|776390400|COMMENT VNTNAME|pEarleyGate 101|COMMENT VNTAUTHORNAME|Pikaard Lab|FEATURES Location/Qualifiers misc_feature 1..25 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 111..363 /label=MAS terminator /note="mannopine synthase terminator" CDS complement(373..924) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" promoter complement(930..1310) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" promoter 2306..2651 /label=35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" protein_bind 2663..2782 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 2812..2842 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 2896..3555 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" CDS 3897..4202 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." protein_bind complement(4243..4367) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS 4375..5088 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" CDS 5167..5193 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA terminator 5225..5932 /label=OCS terminator /note="octopine synthase terminator" misc_feature 6176..6200 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 7500..8129 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label=pVS1 StaA CDS 8558..9631 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 9697..9891 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 10235..10375 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(10561..11149) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11236..12030) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin"ORIGIN 1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg 61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat 121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat 181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa 241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc 301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc 361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct 421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg 481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg 541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg 601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga 661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc 721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc 781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat 841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc 901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat 961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt 1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc 1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt 1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga 1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa 1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat 1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg 1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata 1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa 1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac 1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa 1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc 1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt 1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac 1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt 1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga 1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat 1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat 2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt 2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac 2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga 2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata 2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc 2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag 2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg 2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg 2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 2641 tggagaggac acgctcgaga tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa 2701 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg 2761 taaaacacaa catatccagt catattggcg gccgcattag gcaccccagg ctttacactt 2821 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga 2881 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa 2941 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag 3001 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt 3061 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg 3121 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc 3181 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag 3241 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct 3301 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt 3361 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa 3421 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt 3481 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg 3541 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat 3601 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca 3661 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt 3721 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga 3781 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt 3841 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc 3901 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga 3961 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc 4021 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc 4081 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg 4141 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat 4201 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt 4261 tgtgttttac agcattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata 4321 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgcc tagggtgagc 4381 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 4441 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 4501 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 4561 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac 4621 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 4681 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 4741 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 4801 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 4861 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 4921 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 4981 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 5041 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga 5101 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga 5161 tctagatacc catacgatgt tccagattac gcttaattaa gacccgggac tagtccctag 5221 agtcctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc 5281 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg 5341 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt 5401 tcaatttact gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat 5461 tgtgctgaat aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg 5521 ttttattatt acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact 5581 gattacataa atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac 5641 cgagcggcga actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg 5701 gtgagattcc ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt 5761 caacccggtc cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat 5821 ttttttggtg tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa 5881 tcgttgggcg ggtccagggc gaattttgcg acaacatgtc gaggctcagc aggacctgca 5941 ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 6001 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 6061 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca 6121 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca 6181 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa 6241 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc 6301 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg 6361 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca 6421 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta 6481 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct 6541 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact 6601 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg 6661 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag 6721 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga 6781 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg 6841 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga 6901 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc 6961 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac 7021 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga 7081 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc 7141 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg 7201 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag 7261 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat 7321 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct 7381 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt 7441 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca 7501 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc 7561 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc 7621 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca 7681 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga 7741 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg 7801 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc 7861 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc 7921 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc 7981 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg 8041 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg 8101 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag 8161 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc 8221 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca 8281 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga 8341 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag 8401 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc 8461 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct 8521 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg 8581 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca 8641 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc 8701 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag 8761 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg 8821 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg 8881 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg 8941 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct 9001 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg 9061 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga 9121 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa 9181 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta 9241 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg 9301 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta 9361 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg 9421 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga 9481 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta 9541 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct 9601 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc 9661 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa 9721 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg 9781 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta 9841 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg 9901 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg 9961 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg 10021 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc 10081 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 10141 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 10201 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg 10261 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 10321 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 10381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 10441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 10501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 10561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 10621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 10681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 10741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 10801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 10861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 10921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 10981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 11041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 11101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 11161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 11221 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca 11281 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc 11341 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct 11401 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag 11461 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag 11521 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag 11581 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg 11641 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat 11701 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact 11761 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag 11821 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt 11881 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac 11941 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt 12001 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat 12061 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca 12121 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat 12181 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt 12241 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct 12301 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg 12361 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc 12421 cgagctgccg gtcggggagc tgttggctgg ctgg//
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