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| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCMV6-XL3 |
| 质粒类型: | 哺乳动物表达载体；克隆载体；体外转录载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4713 bp |
| 5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
| 3' 测序引物及序列: | hGH pA rev 5’CCAGCTTGGTTCCCAATAGA 3’ |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 货号： | ZY |
| 供应商： | 泽叶生物 |
| 数量： | 20 |
| 英文名： | pCMV6-XL3 |
| 保质期： | 三年 |
| 保存条件： | -20℃低温保存 |
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AF067197 4713 bp DNA circular SYN 01-JUL-1998DEFINITION Cloning vector PCMV6-XL3, complete sequence.ACCESSION AF067197VERSION AF067197.1 GI:3273769KEYWORDS .SOURCE Cloning vector PCMV6-XL3 ORGANISM Cloning vector PCMV6-XL3 other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 4713) AUTHORS Li,X. TITLE Direct Submission JOURNAL Submitted (20-MAY-1998) Origene Technologies Inc., 13 Taft Ct., Rockville, MD 20850, USAFEATURES Location/Qualifiers source 1..4713 /organism="Cloning vector PCMV6-XL3" /mol_type="genomic DNA" /host="Escherichia coli" /db_xref="taxon:77617" regulatory 201..926 /regulatory_class="promoter" /note="CMV" regulatory 953..971 /regulatory_class="promoter" /note="T7 promoter" misc_feature 972..1037 /note="multiple cloning site" regulatory 1038..1058 /regulatory_class="promoter" /note="T3 promoter" regulatory 1069..1690 /regulatory_class="polyA_signal_sequence" /note="human growth hormone" rep_origin 1747..2053 /note="SV40 origin" rep_origin 2463..3135 /note="ColE1" CDS complement(3280..4140) /codon_start=1 /transl_table=11 /product="ampicillin resistance protein" /protein_id="AAC24838.1" /db_xref="GI:3273770" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4273..4685 /note="f1"ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccgcga attcagatct ggtaccgata tcaagcttct agactgcagt 1021 cgacggccaa gtcggcctcc ctttagtgag ggttaatttg tgatcccggg tggcatccct 1081 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc 1141 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta 1201 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct 1261 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct 1321 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg 1381 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat 1441 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat 1501 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttaaaataac 1561 tataccagca ggaggacgtc cagacacagc ataggctacc tggccatgcc caaccggtgg 1621 gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt tgggtccact cagtagatgc 1681 ctgttgaatt gggtacgcgg ccagcttggc tgtggaatgt gtgtcagtta gggtgtggaa 1741 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 1801 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 1861 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 1921 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 1981 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 2041 tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt taattcccta tagtgagtcg 2101 tattaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 2161 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 2221 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 2281 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 2341 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 2401 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 2461 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 2521 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 2581 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 2641 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2701 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 2761 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 2821 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2881 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2941 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 3001 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 3061 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 3121 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 3181 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 3241 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 3301 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 3361 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 3421 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 3481 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 3541 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 3601 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 3661 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 3721 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 3781 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 3841 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 3901 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 3961 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 4021 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 4081 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 4141 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 4201 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 4261 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 4321 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 4381 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 4441 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 4501 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 4561 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 4621 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 4681 acaaaaattt aacgcgaatt ttaacaaaat att//
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