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| 货号： | human N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit |
| 样本： | 血清、血液、血浆、细胞上清、尿液等 |
| 标记物： | 检测指标 |
| 适应物种： | Homo sapiens human |
| 应用： | 科研 |
| 检测方法： | 双抗夹心法 |
| 检测限： | 说明书下载 |
| 供应商： | 名劲生物 |
| 数量： | 现货 |
| 规格： | 96T |
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Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 1hour at 37℃;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
8. Add 50µL Stop Solution. Read at 450nm immediately.
| 上海名劲生物科技有限公司 | 公司账户：上海名劲生物科技有限公司 |
| 联系人：刘鹏13764978708 | 开户银行：招商银行上海闵行支行 |
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