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| 货号： | GN1042 |
| 供应商： | 上海盖宁生物 |
| 数量： | 现货 |
| 英文名： | pGene/v5-His B |
| 保质期： | 三年 |
| 保存条件： | 常温 |
| 规格： | 5ug质粒 |
| 质粒类型: | 哺乳动物表达载体 |
|---|---|
| 启动子: | Gal4-E1b |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4654bp |
| 5' 测序引物及序列: | pGene Fwd: 5'd[CTGCTATTCTGCTCAACCT]3' |
| 载体标签: | 6X His, V5 |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Zeocin (博来霉素) |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| 1042 | pGene/v5-His B | 5ug质粒 | ¥1000.00 |
质粒图谱
载体描述
The GeneSwitch™ System for inducible mammalian expression is ideal for experiments that require the absolute lowest uninduced expression levels. The expression vector pGene/V5-His provides a minimal promoter, GAL4-E1b, consisting of the binding sites for the yeast Gal4 DNA binding protein followed by the TATA sequence from the Adenovirus E1b promoter. Without additional factors, the GAL4-E1b promoter is transcriptionally silent.To activate transcription from the GAL4-E1b promoter, the GeneSwitch™ regulatory protein is expressed from a minimal TK promoter on the pSwitch vector. The GeneSwitch™ protein has three functional domains:
- Gal4 DNA Binding Domain (Gal4-DBD) to bind the regulatory protein to the GAL4-E1b promoter
- Truncated human Progesterone Receptor Ligand Binding Domain (hPR-LBD) that undergoes a conformational change when it binds the progesterone antagonist, mifepristone
温馨提示：不可用于临床治疗。
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