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| 货号： | SL100489-3LL |
| 保存条件： | 4°C |
| 供应商： | 济南美中清水湾生物科技有限公司 |
| 数量： | 现货 大量 |
| 英文名： | GenJet™ In Vitro DNA Tranfection Reagent for 3LL Cells |
| 保质期： | 一年 |
| 规格： | 毫升/支 |
Description
GenJet™ DNA In Vitro Tranfection Reagent for 3LL cell is pre-optimized for transfecting 3LL cells. 3LL also calledLewis lung carcinoma was discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass.
Refer to the following optimal transfection conditions for maximal transfection efficiency on 3LL cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on 3LL Cells. 3LL cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
GenJet™ DNA In Vitro Tranfection Reagent for 3LL cell is pre-optimized for transfecting 3LL cells. 3LL also calledLewis lung carcinoma was discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass.
Refer to the following optimal transfection conditions for maximal transfection efficiency on 3LL cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) | 80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, 18~24 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 88% |
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on 3LL Cells. 3LL cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 24 hours post transfection
Data Sheet
温馨提示：不可用于临床治疗。
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