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| 货号： | SL100489-PC12 |
| 保存条件： | 4°C |
| 供应商： | 济南美中清水湾生物科技有限公司 |
| 数量： | 现货 大量 |
| 英文名： | GenJet™ In Vitro DNA Tranfection Reagent for PC-12 Cell |
| 保质期： | 一年 |
| 规格： | 毫升/支 |
Description
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 660 transfections in 24 well plates or 330 transfections in 6 well plates.
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor. This makes PC12 cells useful as a model system for neuronal differentiation.
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC12 cells. GenJet™ reagent, 1.0 ml, is sufficient for 660 transfections in 24 well plates or 330 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) | ~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection 48 hours EGFP pEGFP-N3 (CMV promoter) 50% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
温馨提示：不可用于临床治疗。
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