• 游客
收藏 | 举报 2018-01-03 10:08   关注:157   回答:2

为什么我的EcoRI酶切总拖带...

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为什么我的EcoRI酶切总拖带

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  • 游客
举报 2018-01-13 17:47

Enzyme looks fine as digestion in lane 1 shows no smears, signals above the band could be trace amount of contaminants. The plasmid DNA used in lane 2 could be sensitive to EcoR I, or the preparation of this plasmid DNA may be damaged.


  • 游客
举报 2018-01-04 15:16

ECORI,有时候会有星活性,你可以看看你的酶,NEB的酶不是HF的是有星活性的