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举报
2010-03-29 04:30
vector preparation is the key.
digest 1-2 ug plasmid, make sure it is completely digested. retry your ligation |
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举报
2010-03-29 22:19
请提供你的载体及插入片段浓度。
另根据个人经验,不要相信菌落pcr,直接做小抽加酶切鉴定 |
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