• 游客
收藏 | 举报 2016-09-01 21:22   关注:188   回答:5

DNA酶切...

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DNA酶切

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  • 游客
举报 2016-09-09 04:13

What kind of DNA is it, plasmid or genomic sample? How many microgram DNA is used in the digestion? You may need to run agarose gel (1%) to examine the digestion.



  • 游客
举报 2016-09-12 03:56
是基因组DNA,体系中DNA扩增产物4ul,凝胶电泳浓度为2.5%
  • 游客
举报 2016-09-08 17:11

Try to cut 5 ug of genomic DNA in 1 x CutSmart buffer and in a fina volumes of 50 ul at 37 C overnight.

Run 20 ul of digested DNA on a 1 % agarose gel in 1 X TAE at 60 V for 2 hours ( for over 10 cm long gel). Take picture under UV light box.




  • 游客
举报 2016-09-06 00:19

这个流程主要的目的是啥呀?是摸酶切条件?还是……

  • 游客
举报 2016-09-12 09:23

NEB的这个酶理论上1ul能够在15min内切开1ug质粒的,所以按你的酶切体系应该能够完全切开

 
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