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举报
2016-09-09 04:13
What kind of DNA is it, plasmid or genomic sample? How many microgram DNA is used in the digestion? You may need to run agarose gel (1%) to examine the digestion. |
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举报
2016-09-12 03:56
是基因组DNA,体系中DNA扩增产物4ul,凝胶电泳浓度为2.5%
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举报
2016-09-08 17:11
Try to cut 5 ug of genomic DNA in 1 x CutSmart buffer and in a fina volumes of 50 ul at 37 C overnight. Run 20 ul of digested DNA on a 1 % agarose gel in 1 X TAE at 60 V for 2 hours ( for over 10 cm long gel). Take picture under UV light box. |
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举报
2016-09-06 00:19
这个流程主要的目的是啥呀?是摸酶切条件?还是…… |
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举报
2016-09-12 09:23
NEB的这个酶理论上1ul能够在15min内切开1ug质粒的,所以按你的酶切体系应该能够完全切开 |
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