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举报
2016-06-04 14:35
The working solution for T4 DNA ligase should contain ATP and salt. In you ligation reaction, PEG and TE buffer could not activate T4 DNA ligase for connecting the vector ans the insert. Try to add 1 ul of 10 x T4 SNA ligase buffer (NEB) for ligation. |
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举报
2016-05-31 03:24
你好，我想问下buffer不能活化T4吗？还是说我的buffer失活了？biffer一般什么情况下失活呢？望解答，谢谢～
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