【求助】GAPDH可以作为胃癌临床样本组织western b..._内参抗体_一抗_抗体类_知道

游客	收藏 \| 举报 2016-01-04 13:10 关注：293 回答：4 【求助】GAPDH可以作为胃癌临床样本组织western b... 已解决悬赏分：0 - 解决时间 2024-11-16 18:37 【求助】GAPDH可以作为胃癌临床样本组织western blot的内参抗体吗？

游客	举报 2016-01-10 22:04 应该可以

游客	举报 2016-01-14 03:38 43retre 应该可以但是有的文献已经报道了其在肿瘤组织中作为内参抗体，应该慎重考虑啊。。。

游客

举报 2016-01-09 20:59

Glyceraldehyde 3-phosphate dehydrogenase, commonly known as GAPDH, catalyzes the sixth step of glycolysis, it also participates in nuclear events such as transcription, RNA binding and transport, DNA replication and repair, as well as apoptosis. Its expression is high and constant in most tissues and cell types, earning the gene and its protein housekeeping status. Because of this, GAPDH is commonly used as a protein loading control in Western blot and internal control for RT-PCR. However, the amount of GAPDH expression can differ between some tissues and its suitability for your experiment should be taken into consideration before selecting this target as a control. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell and tissue types.

游客

举报 2016-01-07 03:08

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China13113
Glyceraldehyde 3-phosphate dehydrogenase, commonly known as GAPDH, catalyzes the sixth step of glycolysis, it also participates in nuclear events such as transcription, RNA binding and transport, DNA replication and repair, as well as apoptosis. Its expression is high and constant in most tissues and cell types, earning the gene and its protein housekeeping status. Because of this, GAPDH is commonly used as a protein loading control in Western blot and internal control for RT-PCR. However, the amount of GAPDH expression can differ between some tissues and its suitability for your experiment should be taken into consideration before selecting this target as a control. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell and tissue types.

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谢谢大神的回复，但是在肿瘤组织中，是否有文献报道其作为内参的质疑呢？

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【求助】GAPDH可以作为胃癌临床样本组织western b...