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GAL4-LUC双元表达系统和酵母双杂交系统有什么区别？... 已解决
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- 解决时间 2025-01-12 20:03
GAL4-LUC双元表达系统和酵母双杂交系统有什么区别？
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举报
2016-01-02 03:24
求回复啊
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举报
2015-12-30 12:01
Different from yeast two hybrid system which screens for protein protein interaction, GAL4-Luc is luciferase reporter system to examine GAL4 dependent luciferase activity. GAL4 is expressed in a form of fusion proteinwhich is GAL4DNA binding domain together with a hoterogenous transcriptional activation domain from a testing gene. In this way, GAL4 DNA binding and luciferase gene transcription is activated by the fusion protein of GAL4-test gene.
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举报
2016-01-05 03:45
好的，非常感谢您
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