GAL4-LUC双元表达系统和酵母双杂交系统有什么区别？..._双杂交系统_克隆和表达_分子类_知道

全部问答分类

游客	收藏 \| 举报 2015-12-27 15:38 关注：252 回答：3 GAL4-LUC双元表达系统和酵母双杂交系统有什么区别？... 已解决悬赏分：0 - 解决时间 2024-12-04 17:16 GAL4-LUC双元表达系统和酵母双杂交系统有什么区别？

游客	举报 2015-12-28 05:21 求回复啊

游客

举报 2015-12-28 00:48

Different from yeast two hybrid system which screens for protein protein interaction, GAL4-Luc is luciferase reporter system to examine GAL4 dependent luciferase activity. GAL4 is expressed in a form of fusion proteinwhich is GAL4DNA binding domain together with a hoterogenous transcriptional activation domain from a testing gene. In this way, GAL4 DNA binding and luciferase gene transcription is activated by the fusion protein of GAL4-test gene.

游客	举报 2015-12-31 13:24 好的，非常感谢您