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游客	收藏 \| 举报 2017-07-18 14:50 关注：195 回答：5 表达载体... 已解决悬赏分：0 - 解决时间 2025-01-05 07:25 表达载体

游客	举报 2017-07-27 01:33 How big is the insert? How many base pairs are there between the two restriction enzyme sites on the pET28a?

游客	举报 2017-07-20 21:48 825bp,pET28a两个酶切位点之间大概是30个bp

游客	举报 2017-07-19 16:49 mpark How big is the insert? How many base pairs are there between the two restriction enzyme sites on the pET28a? 插曲片段是825bp,pET28a两个酶切位点之间大概是30个bp。谢谢

游客

举报 2017-07-18 20:47

If the insaert DNA is generated from PCR, try to make blunt ending then gel purification of the blunt-ended 825 bp PCR product. make blunt end ligation of the 825 bp fragment on some subclone vector, such pUC19 first. Screening subclones and sequencing the positive one. Cut off the insert DNA and then clone it into pET28a.

游客

举报 2017-07-22 23:00

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mpark
If the insaert DNA is generated from PCR, try to make blunt ending then gel purification of the blunt-ended 825 bp PCR product. make blunt end ligation of the 825 bp fragment on some subclone vector, such pUC19 first. Screening subclones and sequencing the positive one. Cut off the insert DNA and then clone it into pET28a.

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好的，谢谢你