【求助】转录因子表达载体能与荧光素酶报告载体共转染吗..._转录表达_克隆和表达_分子类_知道

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游客	收藏 \| 举报 2010-01-01 21:13 关注：244 回答：2 【求助】转录因子表达载体能与荧光素酶报告载体共转染吗... 已解决悬赏分：0 - 解决时间 2024-12-22 13:24 【求助】转录因子表达载体能与荧光素酶报告载体共转染吗

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举报 2010-01-05 21:21

Before you start these experiments, you need to check NCBI data base to see if the transcription factor A interacts with the promoter B. If there is no such kind data available, you need to run DNA gel retardation assay or DNase I assay to make sure that the transcription factor A interacts with promoter B specifically. Your plan is an important cell-based assay to confirm that the regulation of promoter B transcriptional activities by transcription factor A in tissue cultures.

游客	举报 2010-01-07 11:17 Thank mpark very much.