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Your plan is fine. Try to clone the genomic DNA containing the region you are studying, sp	mpark	2017-12-15 16:49
因为我有一个剪接位点的突变, do you mean you have found a mutation in a splicing lement in	mpark	2017-12-13 16:32
Most likely it is machine error. And it does not make sense to read OD260 after T4 DNA lig	mpark	2018-03-15 23:01
The RNA gel picture is fine. Try to make RNA dissolved well before running the gel.	mpark	2018-03-15 23:03
LNA: locked nucleic acid. Try to use the link:https://en.wikipedia.org/wiki/Locked_nucleic	mpark	2018-03-15 23:31
10000nt长度的DNA片段: a gene or a piece of DNA fragment from any gene? The very simple met	mpark	2018-03-15 23:38
而当目的基因为OCT4/SOX2等多能性目的基因时，效率不到10%: how is this 10% rate determined? G	mpark	2018-03-13 13:44
In sample lane the most fast-running bands could be RNA, the very slow running bands at th	mpark	2018-03-15 23:12
Please label the gel picture.	mpark	2018-01-26 00:58
Try to run low melting temperature agarose gel and extract the PCR fragments from the gel	mpark	2012-06-15 12:13
You can not try to compare levels of mRNA vis levels of the mRNA translated products. By t	mpark	2018-03-16 14:03
Please show the RT-qPCR and WB results.	mpark	2018-03-15 23:33
In step B, are you sure that for 10 cm dish only 10 ml medium isadded? How many cells are	mpark	2016-04-06 23:43
Try to spot the DNA samples in small volumes (less than 50 ul) on the membrane with the ai	mpark	2011-11-04 23:04
Try to make a 1% long gel and load each well with 0.2-0.4 ug of digested plasmid for 10 we	mpark	2015-10-26 16:03
How big the T vector is? Do you have any gel picture?	mpark	2015-10-25 00:41
Please download the attached file for details:AJMB-7-85.pdf(380.42k)	mpark	2018-03-16 23:28
质粒浓度还是很低呢？what is the concentration? Try to grow 1000 ml bacterial cultures in l	mpark	2018-03-16 23:31

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